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Old 08-24-2017, 11:49 AM   #1
Location: U.S.

Join Date: May 2017
Posts: 21
Default troubleshooting agilent bioanalyzer error

I ran an RNA 6000 nano chip with RNA samples in all 12 wells, some randomly came back flagged with error: unexpected signal in fast region. Is there any way to troubleshoot this after the fact to get an accurate RIN number?
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Old 08-24-2017, 12:44 PM   #2
Location: Illinois

Join Date: Oct 2014
Posts: 42

You should be able to manually adjust the fast region signal allowance under the advanced settings and this will clear the error, allowing for a RIN to be called.
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Old 10-31-2018, 01:39 PM   #3
Location: US

Join Date: Jan 2016
Posts: 14

Agilent tech support is really helpful with fixing these problems, if you are unsure how to do so. (Via phone or email them the run file)
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bioanalyzer, library construction, quality control, sample prep

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