Short read 1 on a Nova

GW_OK · 08-22-2019, 01:38 PM

Anyone tried a read 1 less than 25 cycles on a Nova? We've done it before on Miseq and Nextseq but not on Nova. I'm wondering if there's anything weird about the patterned flowcell that would make it not work....

GenoMax · 08-22-2019, 01:59 PM

I don't immediately recall if we have done one on NovaSeq but have done so on patterned FC on HiSeq 4000. As long as you keep in mind that the quality scores are not properly calibrated until after cycle 25 it should work.

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08-22-2019, 01:38 PM	#1
GW_OK Senior Member Location: Oklahoma Join Date: Sep 2009 Posts: 411	Short read 1 on a Nova Anyone tried a read 1 less than 25 cycles on a Nova? We've done it before on Miseq and Nextseq but not on Nova. I'm wondering if there's anything weird about the patterned flowcell that would make it not work....

08-22-2019, 01:59 PM	#2
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,080	I don't immediately recall if we have done one on NovaSeq but have done so on patterned FC on HiSeq 4000. As long as you keep in mind that the quality scores are not properly calibrated until after cycle 25 it should work.