Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
MSA on large scale of sequences? mastercoder Bioinformatics 23 01-05-2017 09:54 AM
Scale Up or Down Reaction Volumes? Nighthawkrao77 Sample Prep / Library Generation 1 03-19-2015 02:28 PM
CummerBund error scale plots Mokinhas Bioinformatics 1 12-04-2013 03:19 AM
Large scale cuffdiff analysis DerSeb Bioinformatics 2 04-03-2013 10:23 AM
Scale E20 ? soleulloa SOLiD 1 09-22-2011 04:26 AM

Thread Tools
Old 09-14-2017, 02:32 PM   #1
Junior Member
Location: California

Join Date: Sep 2017
Posts: 1
Default Scale

Hi! My lab wants to do a pilot about skin microbiome (which I am not an expert) and I need to request a quote for V1-V3 primers, the company asked me which scale for synthesis I want the oligos.
According to some websites 25nmil seems reasonable.
Any thoughts to help me?

thank you

Last edited by junoguti; 09-14-2017 at 02:36 PM.
junoguti is offline   Reply With Quote
Old 09-15-2017, 01:59 AM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,220

25 nmole with standard desalting is OK and should last 100s of reactions. Depending on oligo length or modifications, oligos might need PAGE or HPLC purification which would require higher synthesis concentrations and this differ among manufacturers.
nucacidhunter is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:19 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO