Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
Repbase needed k-gun12 Bioinformatics 12 10-31-2014 02:42 PM
Newbie...Help needed!! Beta1 Sample Prep / Library Generation 1 01-23-2012 09:21 AM
Help needed smjazayeri Introductions 0 06-16-2011 03:08 PM
What is the minimum coverage needed? Annettet Illumina/Solexa 0 03-22-2011 02:41 AM
Hello and help needed :) from Indiana University hpeitz Bioinformatics 2 07-17-2010 12:01 PM

Thread Tools
Old 05-12-2011, 08:07 PM   #1
Location: ny

Join Date: Jul 2009
Posts: 23
Default clogged HiSeq, help needed


Our HiSeq2000 is experiencing a lot of fluidic problem recently. We will lose a lane or 2 during the run due to a clogged pump or maybe air bubble?. The Illumina engineer doesn't seem to have a fix except for just flushing the system to remove the clog.

It is really hurting our throughput at this moment as most of the time there is only 1 working flowcell...

Any suggestion please?
silin284 is offline   Reply With Quote
Old 05-13-2011, 11:09 AM   #2
Senior Member
Location: Oklahoma

Join Date: Sep 2009
Posts: 411

I lost a lane on my hiseq a month or so ago. Needed one of the valves on top of the syringes replaced.

Depending on how adventurous you are you can take the face plate off the syringe bay (a single screw behind the reagent chiller door and it slides right out) and swap the inputs of two different syringes. That'd let you know if it's those valves or not at least. I troublshot a GA2x that way, the engineer said it saved him time since he already knew what he had to replace.
GW_OK is offline   Reply With Quote
Old 05-16-2011, 02:50 PM   #3
Junior Member
Location: n/a

Join Date: Apr 2011
Posts: 2

This has been happening to us a lot, and there's only so much a user can do. Keep up-to-date with maintenance washes, and do extra water washes if it's not in use for a period of time.
We've had 2 separate machines independently get a brown precipitate in their fluidics systems that have clogged them up. We have no idea if it's coming from outside or inside the system, but if someone else has had it, please post! That way we'd at least have a better idea if it was Illumina reagents/chem/hardware or from our specific lab.
We've reached the point that if there's any indication of a clog, we attempt a little trouble shooting with gaskets, seal and water flushes, then tell our FSE.
rabbitstew is offline   Reply With Quote
Old 05-27-2011, 01:16 AM   #4
Location: Germany

Join Date: Feb 2010
Posts: 32

We had serverall issuses of cloggage since we got our HiSeq.
Ask your FSE for a adapter to de-clog yourself, which works by opening the front panel, connecting the correct lanes tubing to a syringe and flush it with water manually. Be paranoid about what you use for washing the HiSeq. Make sure there is no debris in water or in the sodium hydroxide.
Baseless is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 10:19 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO