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Old 06-30-2011, 07:55 AM   #1
glassfish
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Default Sample purification question

Hi all,

I am going to drop off my first batch of DNA samples for sequencing next week. I plan to do a paired-end 101 sequencing of GAII. I sheared the DNA to 200bp by a Covarit. I plan to purify the DNA fragments with QIAquick gel extraction kit, but the technician from our core facility told me I don't need to purify them. I just want to check whether it is normal... Thanks a lot!
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Old 07-05-2011, 11:22 AM   #2
jjenrette
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It is normal. You do not need to purify. The library prep protocol will contain many purification steps along the way.
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Old 07-06-2011, 01:10 AM   #3
protist
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There is no need to gel purify - we do run the fragmented material through a Qiagen PCR column but that is only to concentrate the sample.
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