SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > SOLiD



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Performance comparison of exome DNA sequencing technologies. Newsbot! Literature Watch 0 02-02-2012 11:20 PM
HELP,why consider reads with 5' adaptor as contaminants? Euler Bioinformatics 0 01-14-2010 10:50 PM
PubMed: Comparison of next generation sequencing technologies for transcriptome chara Newsbot! Literature Watch 0 08-04-2009 05:00 AM
Adaptor technologies comparison casual_seqs Sample Prep / Library Generation 0 06-17-2009 02:21 AM
PubMed: A Comparison of Global Gene Expression Measurement Technologies in Arabidopsi Newsbot! Literature Watch 0 07-17-2008 05:02 AM

Reply
 
Thread Tools
Old 06-15-2009, 07:45 AM   #1
casual_seqs
Junior Member
 
Location: paris

Join Date: Jun 2009
Posts: 5
Default Adaptor technologies comparison

Hello,
I am interested in the comparison/respective advantages and weak points of the various adapters technologies, from the point of view of in-vitro genomic libraries construction and amplification, the avoidance of artifacts, and ease of preparation.

1) anyone with a synthetic view on this (including the adapter technologies of Solexa, Illumina, Solid, etc...) ?
I am interested in the finicky details also (eventual exo protection, eventual 3'-end and/or 5'-end modification, etc...)

2) about the Solid adaptors:(see also http://seqanswers.com/forums/showthread.php?t=588)

I have read in this forum (http://seqanswers.com/forums/showthread.php?t=198, sci_guy), that the solid adaptors are non-phosphorylated, so that P1-P1, P2-P2, or P1-P2 dimers are not formed at the ligation step; (by the way, are the 3'TT-overhangs efficient at avoiding ligation to genomic DNA ?)
Also, for the amplification steps, the targets of the primers are generated by nick translation.
Those features appears clever; Does this technique readily outperforms the competitor's adapters technologies ?

3) Would someone have the detailled solid protocol for library prep at hand (under electronic form), that could be forwarded/downloaded/or pasted in this thread?
casual_seqs is offline   Reply With Quote
Old 06-17-2009, 02:44 AM   #2
casual_seqs
Junior Member
 
Location: paris

Join Date: Jun 2009
Posts: 5
Default

I transferred this post to
http://seqanswers.com/forums/showthread.php?p=5977
thanks to post answers there.
casual_seqs is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 08:06 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO