SEQanswers

Go Back   SEQanswers > Applications Forums > RNA Sequencing



Similar Threads
Thread Thread Starter Forum Replies Last Post
Validate SNP calls from NGS sequencing data with genotying array bioinf newbie Bioinformatics 1 09-20-2012 09:05 PM
Quetion about DGE tanger.zhang RNA Sequencing 0 05-01-2012 08:53 PM
qPCR to validate/quantify Nextera library mikmz Sample Prep / Library Generation 0 12-14-2011 12:26 AM
Any way to validate BFAST index file? feederbing Bioinformatics 2 09-08-2011 02:21 PM
how to validate SNPs and Indels after assembly? sulicon Bioinformatics 9 02-25-2011 04:27 AM

Reply
 
Thread Tools
Old 02-12-2013, 11:02 AM   #1
seb330
Junior Member
 
Location: Pennsylvania

Join Date: Oct 2012
Posts: 5
Default qPCR to validate DGE

I'm designing some qPCR reactions to test some candidate genes before we get our RNA-seq results back. Does anyone have a good suggestion for a control gene to use for bacterial samples? I've heard that just using 16S rRNA is problematic because it is so abundant. Also, since rRNA shouldn't be in my RNA-seq reactions, I'm hesitant to use that as a control. I've seen a lot of people use recA. Is this common, or are there other suggestions?
seb330 is offline   Reply With Quote
Old 05-07-2013, 06:56 PM   #2
Pengfei Liu
Member
 
Location: Beijing

Join Date: Nov 2012
Posts: 16
Default

Do you have any idea on this issue now? I am also concerned with this problem.
Pengfei Liu is offline   Reply With Quote
Old 05-08-2013, 07:56 AM   #3
bernardo_bello
Member
 
Location: Spain

Join Date: May 2012
Posts: 51
Default

Quote:
Originally Posted by Pengfei Liu View Post
Do you have any idea on this issue now? I am also concerned with this problem.
I'm also in search of bacterial qPCR standards.

Bernardo
bernardo_bello is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:49 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO