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Old 05-07-2013, 03:56 AM   #1
mhadidi2002
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Default BLAST best hit?

Hello,

I would like to ask about the standalone BLAST output best hit. Is it the first match for a given query sequence is always the best hit? As long as I know, it is sorted based on the E-value, the best match (with the lowest E-value) comes in the first, followed by the others. Right?

Best Regards,
BioinfGuy
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Old 05-07-2013, 10:32 AM   #2
rhinoceros
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The lowest e-value doesn't necessarily always mean 'the best', but in most cases, yes. Even more so if you mask regions of low complexity..
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