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Old 03-04-2014, 07:18 AM   #1
taqpol2011
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Default Dual SPRI cleanup with Chipseq

Hello everyone,
Has anyone heard of recommendations to not run a dual bead clean up after adapter ligation with low input libraries? Does this work?
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Old 03-04-2014, 10:33 AM   #2
kwaraska
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Yes-we run only single sided SPRI. It is mostly because you cannot control the shearing in ChIP so the sizes may be different. You don't want to lose any sites.
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