Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
AMPure XP vs SPRI beads Tali7 Sample Prep / Library Generation 19 07-12-2017 11:13 AM
repeating chipseq or chipseq with another antibody hawainpanda Sample Prep / Library Generation 2 03-12-2015 03:56 PM
Indexing ChIPseq libraries using Illumina's TruSeq and ChIPseq kits Alex Clop Epigenetics 6 11-08-2012 11:07 AM
SPRI bead for PCR cleanup in illumina pair end library preparation Berkeley2010 Illumina/Solexa 2 10-20-2010 12:46 PM
Wiki cleanup? krobison Wiki Discussion 3 05-02-2010 02:12 PM

Thread Tools
Old 03-04-2014, 07:18 AM   #1
Junior Member
Location: US

Join Date: Feb 2013
Posts: 1
Default Dual SPRI cleanup with Chipseq

Hello everyone,
Has anyone heard of recommendations to not run a dual bead clean up after adapter ligation with low input libraries? Does this work?
taqpol2011 is offline   Reply With Quote
Old 03-04-2014, 10:33 AM   #2
Senior Member
Location: Boston,MA

Join Date: Nov 2008
Posts: 122

Yes-we run only single sided SPRI. It is mostly because you cannot control the shearing in ChIP so the sizes may be different. You don't want to lose any sites.
kwaraska is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:02 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO