SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Deep sequencing of microRNA precursors reveals extensive 3' end modification. Newsbot! Literature Watch 0 08-19-2011 11:02 PM
PubMed: Deep sequencing reveals distinct patterns of DNA methylation in prostate canc Newsbot! Literature Watch 0 07-05-2011 06:40 AM
PubMed: Deep mRNA Sequencing for In Vivo Functional Analysis of Cardiac Transcription Newsbot! Literature Watch 0 04-03-2010 02:02 AM
PubMed: Next-generation sequencing reveals complex relationships between the epigenom Newsbot! Literature Watch 0 10-13-2009 02:00 AM
PubMed: Deep-sequencing of plant viral small RNAs reveals effective and widespread ta Newsbot! Literature Watch 0 08-12-2009 05:00 AM

Reply
 
Thread Tools
Old 04-03-2010, 02:02 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,443
Default PubMed: mRNA deep sequencing reveals 75 new genes and a complex transcriptional lands

Syndicated from PubMed RSS Feeds

mRNA deep sequencing reveals 75 new genes and a complex transcriptional landscape in Mimivirus.

Genome Res. 2010 Apr 1;

Authors: Legendre M, Audic S, Poirot O, Hingamp P, Seltzer V, Byrne D, Lartigue A, Lescot M, Bernadac A, Poulain J, Abergel C, Claverie JM

Mimivirus, a virus infecting Acanthamoeba, is the prototype of the Mimiviridae, the latest addition to the nucleocytoplasmic large DNA viruses. The Mimivirus genome encodes close to 1000 proteins, many of them never before encountered in a virus, such as four amino-acyl tRNA synthetases. To explore the physiology of this exceptional virus and identify the genes involved in the building of its characteristic intracytoplasmic "virion factory," we coupled electron microscopy observations with the massively parallel pyrosequencing of the polyadenylated RNA fractions of Acanthamoeba castellanii cells at various time post-infection. We generated 633,346 reads, of which 322,904 correspond to Mimivirus transcripts. This first application of deep mRNA sequencing (454 Life Sciences [Roche] FLX) to a large DNA virus allowed the precise delineation of the 5' and 3' extremities of Mimivirus mRNAs and revealed 75 new transcripts including several noncoding RNAs. Mimivirus genes are expressed across a wide dynamic range, in a finely regulated manner broadly described by three main temporal classes: early, intermediate, and late. This RNA-seq study confirmed the AAAATTGA sequence as an early promoter element, as well as the presence of palindromes at most of the polyadenylation sites. It also revealed a new promoter element correlating with late gene expression, which is also prominent in Sputnik, the recently described Mimivirus "virophage." These results-validated genome-wide by the hybridization of total RNA extracted from infected Acanthamoeba cells on a tiling array (Agilent)-will constitute the foundation on which to build subsequent functional studies of the Mimivirus/Acanthamoeba system.

PMID: 20360389 [PubMed - as supplied by publisher]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:16 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO