Hi all,
This is my first post here, so I am taking the opportunity to thank all the people who make this forum live.
It is possible to find read counts from CCLE here :
I would like to use these data to find genes involved in resistance to treatment.
I would like two group these cell lines in "sensitive" and "resistance" and test for the DEGs.
However there is no technical replicats for these data.
Let's say I have 32 cell lines in the "resistant" group and 32 cell lines in the "sensitive" group.
Can I perform a DEG analysis with edgeR ?
Can the 32 cell lines in each group be considered as replicats ?
Do you think my approach is valid ?
Thank you very much.
This is my first post here, so I am taking the opportunity to thank all the people who make this forum live.
It is possible to find read counts from CCLE here :
I would like to use these data to find genes involved in resistance to treatment.
I would like two group these cell lines in "sensitive" and "resistance" and test for the DEGs.
However there is no technical replicats for these data.
Let's say I have 32 cell lines in the "resistant" group and 32 cell lines in the "sensitive" group.
Can I perform a DEG analysis with edgeR ?
Can the 32 cell lines in each group be considered as replicats ?
Do you think my approach is valid ?
Thank you very much.