Hi all,
Several people have posted questions on this forum about TruSeq Custom Amplicon (TSCA) or the TruSeq Amplicon - Cancer Panel (same tech?) but nobody seems to have the answers (or are not willing to share them).
Illumina's website states their TruSeq Amplicon - Cancer Panel works on FFPE (well, if your FFPE DNA samples pass their QPCR test, and then only "around" 80% will work). Separately, Illumina Tech Support stated to me they did not recommend using FFPE DNA with TSCA (though that was in March, perhaps things have changed).
My question is - has anyone used TruSeq Custom Amplicon or the TruSeq Amplicon Cancer Panel on DNA from FFPE samples, and if so - how did it perform? The lack of information may be telling in itself, but someone must be willing/able to share...
Regards,
HedleyC
Several people have posted questions on this forum about TruSeq Custom Amplicon (TSCA) or the TruSeq Amplicon - Cancer Panel (same tech?) but nobody seems to have the answers (or are not willing to share them).
Illumina's website states their TruSeq Amplicon - Cancer Panel works on FFPE (well, if your FFPE DNA samples pass their QPCR test, and then only "around" 80% will work). Separately, Illumina Tech Support stated to me they did not recommend using FFPE DNA with TSCA (though that was in March, perhaps things have changed).
My question is - has anyone used TruSeq Custom Amplicon or the TruSeq Amplicon Cancer Panel on DNA from FFPE samples, and if so - how did it perform? The lack of information may be telling in itself, but someone must be willing/able to share...
Regards,
HedleyC
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