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Old 12-22-2009, 06:50 PM   #1
lvxiaobao
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Location: guangzhou city ,guangdong province ,china

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Default help: time of adapter ligation

hello everyone , i'am sample prepation of mRNA, and i have a question about adapter ligation to the dsCDNA:
the protocol of illuminate write: incubate the sample at room temperature for 15 minutes.
as we know we usually make a ligation action overnight, i wonder if 15 minutes is enough for ligation reaction?
thanks! hopes your join!
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Old 12-24-2009, 07:37 AM   #2
floor121
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If you use the illumina kit to construct library, 15 min is enough for adaptor ligation; if not, the ligation time should be optimized.

Last edited by floor121; 08-16-2011 at 11:41 AM.
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