SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Pooling TruSeq (single index) and Nextera XT (dual index) in one MiSeq run? Carosmile Sample Prep / Library Generation 1 05-16-2017 02:13 PM
MiSeq - minimum index diversity? JBKri Illumina/Solexa 10 03-27-2016 07:49 AM
MiSeq v3 kit for high diversity vs. low diversity libraries phyllosphere Illumina/Solexa 4 12-23-2015 01:29 PM
MiSeq Index Read PTW Illumina/Solexa 4 01-23-2013 10:24 AM
Miseq index reads missing scotoma Illumina/Solexa 4 11-06-2012 02:12 PM

Reply
 
Thread Tools
Old 10-19-2017, 06:48 AM   #1
kerryp
Member
 
Location: Scotland

Join Date: Apr 2015
Posts: 16
Default index diversity on MiSeq

Can I run 2-3 index combinations on MiSeq? I'd be using Nextera XT DNA sample preparation index kit and MiSeq v2 reagent kit. If I select index pairs with maximum diversity, might it be safe to try?
What would insufficient index diversity look like, and is the data retrievable in the worst case scenario?
Thanks
kerryp is offline   Reply With Quote
Old 10-19-2017, 10:22 AM   #2
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 6,546
Default

If you use the standard indexes it should be fine. Illumina has a white paper somewhere that recommends specific combinations of indexes depending on number of samples you are pooling.
GenoMax is online now   Reply With Quote
Old 10-19-2017, 02:59 PM   #3
nucacidhunter
Senior Member
 
Location: Iran

Join Date: Jan 2013
Posts: 1,074
Default

And here is the link for Nextera low plex pooling guide:

https://www.illumina.com/documents/p...guidelines.pdf
nucacidhunter is offline   Reply With Quote
Reply

Tags
dual indexing, index diversity, miseq v2, nextera xt indices

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 01:11 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO