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Old 09-29-2017, 03:27 AM   #1
Mizraelson
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Default How much MiSeq sequencing reagent is being used per each cycle.

Hi everyone. I have some thoughts to use leftovers after several MiSeq runs to perform one extra run (I do have an extra flowcell, but reagents are needed). So my question is how much reagent is used by MiSeq per each cycle? Does anyone now that or have an experience in using the rest of the reagent after a run?

Last edited by Mizraelson; 09-29-2017 at 03:39 AM.
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Old 09-29-2017, 05:00 AM   #2
GenoMax
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I understand being frugal is a good thing but trying to re-use reagents that are several days old and stayed out at 6-8 degrees in the machine does not seem like a wise choice unless you want to do this purely as an experiment (you will need to pool reagents from multiple runs unless you have a large amount left over due to a short run with a long run kit).

Generally all kits have enough extra reagents to do 10-15 cycles (to account for index reads) as I recall.
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Old 09-29-2017, 06:17 AM   #3
Mizraelson
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Sure, that's purely an experiment. Thats exactly what I want to do - to pool reagents from multiple runs and I do sometimes see there a lot left in the cartridge. So is there a way to more or less predict how much reagent (by volume) is needed for one cycle in order to extrapolate.
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Old 09-29-2017, 07:15 AM   #4
GenoMax
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Someone with better experimental expertise than me will have to answer that.
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Old 09-29-2017, 12:34 PM   #5
kcchan
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A lot of the leftover reagents is actually water from the template rinse and back flush. Probably not a good idea to re-use them.
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