I am trying to replicate a protocol established in the labs of our collaborator. The problem is they have provided me with the adapter sequences for barcoding multiple samples but did not provide any further information. Nor are they responding to emails.
I have always used Illumina generated indexes (nextera) so can't figure out what are the index sequences in these adapters. Any help will be appreciated.
The sequences are:
a1: ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT GTC ATG A*T
a2: /5Phos/TCA TGA CAG ATC GGA AGA GCG TCG TGT AGG GAA AGA GTG T
a3: ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT ACA TCT C*T
a4: /5Phos/GAG ATG TAG ATC GGA AGA GCG TCG TGT AGG GAA AGA GTG T
* = phosphorothiorate bond
Which are the index sequences to be used for demultiplexing the samples, once the run is over?
I have always used Illumina generated indexes (nextera) so can't figure out what are the index sequences in these adapters. Any help will be appreciated.
The sequences are:
a1: ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT GTC ATG A*T
a2: /5Phos/TCA TGA CAG ATC GGA AGA GCG TCG TGT AGG GAA AGA GTG T
a3: ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT ACA TCT C*T
a4: /5Phos/GAG ATG TAG ATC GGA AGA GCG TCG TGT AGG GAA AGA GTG T
* = phosphorothiorate bond
Which are the index sequences to be used for demultiplexing the samples, once the run is over?
Comment