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  • PCR program for Sureselect libraries

    Hello,
    After hybridisation in the Sureselect protocol, there is a PCR amplification. What program do you use for this step?

    The program currently in use is:
    98oC at 2 mins
    11 cycles of 98oC at 30s/57oC at 30s/72oC at 1 min
    72oC at 10 mins
    hold at 4oC

    The capture size is over 1.5Mb.

    Thanks for your help,
    Anna.

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