Has anyone experienced that the sequences generated after the capture are mostly those not included in the capture??
I have just looked at 1/10 individuals so far, but none of my selected genes have any reads mapping to the exons, in some cases there are some reads in the introns... I am thinking all my precious sequences are still stuck to the beads
Any thoughts?
I have just looked at 1/10 individuals so far, but none of my selected genes have any reads mapping to the exons, in some cases there are some reads in the introns... I am thinking all my precious sequences are still stuck to the beads
Any thoughts?
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