I'm using the Tru-seq Stranded mRNA Sample Preparation kit and I made the mistake of not removing the RNA from the RNA Purification Beads before starting the First strand synthesis and then second strand synthesis. The only step I missed was removing the beads, I added everything else and ran the incubations required for the first and second strand synthesis. Is everything okay to continue or will I have to start over?
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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03-22-2024, 06:39 AM -
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