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  • NGS analysis, questions to ask ...

    Before taking up NGS data for analysis interesting questions raised early on can pretty much foster a successful analysis through. This ensures no communication breakdowns take place between scientist on both sides of the NGS story, the sequencing facility and the computational facility.

    As a bioinformatician it is interesting to me to have information on the type of experiment (DNA seq, RNA seq, assembly...etc), data (single end, paired end), platforms and throughput, depth and coverage of reads, samples/patients naming conventions, encoding, replicates, study design (whole genome, whole exon..etc), adaptor files if any. And foremost; on how far my capacity (load/experience/enthusiasm) can lead me.

    My short list maybe missing more interesting question so what are your expectations before accepting data for analysis depending on the type of experiment ?

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  • seqadmin
    Current Approaches to Protein Sequencing
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    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
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    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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