Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • problem in using ERANGE

    hello everyone,

    I am working on SOLiD platform and want to use SOLiD data for RNAseq analysis. For that i am trying to utilize bowtie for mapping an ERANGE for RPKM. For bowtie mapping we need expanded genome. i am trying to utilize getsplicefa.py of ERANGE to get splice file. but while doing this is am getting the following error:

    psyco not running
    /share/apps/ERANGE/project/genome/commoncode/getsplicefa.py: version 3.2
    43180
    369006
    could not get chr3:72692980-72695247
    could not get chr3:72695415-72695619
    could not get chr3:72695669-72704339
    could not get chr3:72704428-72707527
    could not get chr3:72707652-72708182
    could not get chr3:72708239-72708321
    could not get chr3:72708406-72711003
    could not get chr3:72711152-72712888
    could not get chr3:72713005-72713089
    contt...

    Any help is appreciable

    Saha

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
31 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
53 views
0 likes
Last Post seqadmin  
Working...
X