Hi all,
Has anyone used the large, presumably mRNA-enriched fraction from the Mirvana protocol for TruSeq library prep and Illumina mRNA-seq? Bioanalyzer tells me there's 0% ribosomal contamination in my large RNA fraction from the Mirvana prep, but I'm wondering if I should still do the poly-A selection beads step with the TruSeq kit or not.
Thanks for your input,
Matt
Edit: I just realized that the size range of these "mRNA" samples looks huge; see attached Bioanalyzer gel, left side samples. Perhaps a lot of unspliced pre-mRNAs? So I should probably still do poly-A selection...
Has anyone used the large, presumably mRNA-enriched fraction from the Mirvana protocol for TruSeq library prep and Illumina mRNA-seq? Bioanalyzer tells me there's 0% ribosomal contamination in my large RNA fraction from the Mirvana prep, but I'm wondering if I should still do the poly-A selection beads step with the TruSeq kit or not.
Thanks for your input,
Matt
Edit: I just realized that the size range of these "mRNA" samples looks huge; see attached Bioanalyzer gel, left side samples. Perhaps a lot of unspliced pre-mRNAs? So I should probably still do poly-A selection...
Comment