Hi Everybody!
Most of us use Bioanalyzer Chips to check the libraries (and RNA if working with it) but unfortunately Agilent website is not mentioning much details about how does this appliance work.
I am extremely interested (due to strange results) what is the denaturation method applied for RNA sample? is there urea (doubtfully) included? or is it maybe only very high temperature in low ionic conditions?
Another question (less important) is what dye is used to stain nucleic acids for detection?
If any of you was able to find answers to this simple questions I would really appreciate sharing this knowledge.
Lukasz
Most of us use Bioanalyzer Chips to check the libraries (and RNA if working with it) but unfortunately Agilent website is not mentioning much details about how does this appliance work.
I am extremely interested (due to strange results) what is the denaturation method applied for RNA sample? is there urea (doubtfully) included? or is it maybe only very high temperature in low ionic conditions?
Another question (less important) is what dye is used to stain nucleic acids for detection?
If any of you was able to find answers to this simple questions I would really appreciate sharing this knowledge.
Lukasz
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