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Old 06-27-2021, 07:34 PM   #1
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Location: Malaysia

Join Date: Jun 2021
Posts: 1
Default Mauve 'std::ios_base::failure'

I'm using a linux server to run progressiveMauve to align 2 genomes of the same species for synteny analysis. But I kept encounter this issue:

$ progressiveMauve --output=output.xmfa EtH_Genome.fasta NSN_Genome.fasta
Storing raw sequence at /tmp/rawseq452439.000
Sequence loaded successfully.
EtH_Genome.fasta 51859607 base pairs.
Storing raw sequence at /tmp/rawseq452439.001
Sequence loaded successfully.
NSN_Genome.fasta 128363032 base pairs.
Using weight 19 mers for initial seeds
Creating sorted mer list
Error creating sorted mer list
terminate called after throwing an instance of 'std::ios_base::failure'
what(): failed getting file size: Value too large for defined data type

Does anyone know how can I resolve this issue? Do I have to compress my fasta file before running it?
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