SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > 454 Pyrosequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Agilent Bioanalyzer alternatives? rmetz Sample Prep / Library Generation 15 09-01-2014 09:46 PM
Anyone has protocol for Ion PES protocol? marcowanger Ion Torrent 1 01-18-2012 08:28 PM
Alternatives to sfffile & sffinfo Xterra 454 Pyrosequencing 5 06-28-2010 07:09 AM
illumina alternative v1.5 protocol for small rna seq vs. the standard protocol ik76 Sample Prep / Library Generation 1 03-25-2010 02:24 PM
PubMed: Methods for generating shotgun and mixed shotgun/paired-end libraries for the Newsbot! Literature Watch 0 04-11-2009 05:40 AM

Reply
 
Thread Tools
Old 04-05-2011, 08:08 AM   #1
Raj
Member
 
Location: UK

Join Date: Jan 2009
Posts: 15
Default Alternatives to Roche Shotgun and PE protocol

Hi,
I hope you can help or give some pointers in the right direction. we've been working with Junior recently and have tried genome sequencing using the 8 kb pair end, it worked, which was nice but it was a lot of work and took a long time.

I was wondering if anybody has come across alternative protocols that may simplify or speed up either the pair end protocol or the shot gun protocol.

I have come across the cambio enzyme chemistry alternative and the beckman SPRI works robot for 454, which both look promising for the shot gun alternative?!

Any other suggestions are more than welcome!!!!

Thanks in advance ...
Raj is offline   Reply With Quote
Old 04-08-2011, 04:09 AM   #2
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 1,850
Default

Roche's "paired end" libraries are what would be called "mate pair" on the Illumina or SOLiD platforms. These are quite challenging libraries to construct.

However the shotgun libraries are not too bad. Are you using the "Rapid" protocol?

Epicentre has a kit that might be what you are looking for:

http://www.epibio.com/nextera/nextera.asp

--
Phillip
pmiguel is offline   Reply With Quote
Reply

Tags
454, junior, protocol

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:50 AM.


Powered by vBulletin® Version 3.8.6
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.