Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • inesdesantiago
    Member
    • Jan 2009
    • 44

    bowtie - windows instalation

    Hello.
    I am trying Bowtie. It seams very fast and efficient!
    However I have a minor problem in my installation (windows XP)

    1 - I downloaded Bowtie from the link

    file: bowtie-0.9.8.1-bin-win32.zip

    2 - Unzipped to C:/bowtie

    3 - Test it:

    bowtie -t e_coli reads/e_coli_1000.fq e_coli.map

    Bowtie website says:
    "This run calculates the same alignments as the previous run, but the alignments are written to e_coli.map (the final argument) rather than to the screen. Also, the -t option instructs Bowtie to print timing statistics, which will look something like this (times will obviously differ):

    Time loading forward index: 00:00:00
    Time loading mirror index: 00:00:00
    Seeded quality full-index search: 00:00:01
    Reported 699 alignments to 1 output stream(s)
    Time searching: 00:00:01
    Overall time: 00:00:01

    "

    However in my computer the output stops after the first two lines and stays like that forever if I let him.
    Something like this:

    Time loading forward index: 00:00:00
    Time loading mirror index: 00:00:00


    When I check the file with the results (e_coli.map) it seams complete. With the 699 lines...

    I am not sure if I should have done some compilation or installation of Bowtie before starting the runs. I merely downloaded it into a folder and execute the command..
    Any ideas or thoughts?

    Thanks!
    Ines
  • Ben Langmead
    Senior Member
    • Sep 2008
    • 200

    #2
    Hi Ines,

    I am aware of this problem and am going to take a look at it soon. It most likely has something to do with the MinGW pthreads library.

    No, you don't need to do any compilation or installation beyond just unzipping the package - the error is my fault.

    Thanks,
    Ben

    Comment

    • doxologist
      Member
      • Jan 2009
      • 96

      #3
      bowtie runs well in cygwin.
      would a windows installation be preferable over a linux one in cygwin?

      Comment

      • Asifullah
        Junior Member
        • Aug 2010
        • 5

        #4
        dear All,

        I have recently joint the forum, it is interesting. Actually i want to use Bowite Tool for 72 bp fragments of illumina read (microbail genome) assembling via windowXp based operating system. could any one guide me from very basics of window based installation and operation of this tool. Previously i have assemble a genome with DNAStar tool. Any body could help please. ? his/her help will be highly appreciated.

        Asifullah Khan

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Nine Things a Sample Prep Scientist Thinks About Before Sequencing
          by SEQadmin2


          I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

          Here are nine questions we think about, in roughly the order they matter, before...
          06-18-2026, 07:11 AM
        • SEQadmin2
          From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
          by SEQadmin2


          Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


          The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
          ...
          06-02-2026, 10:05 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Yesterday, 11:10 AM
        0 responses
        7 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-17-2026, 06:09 AM
        0 responses
        42 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-09-2026, 11:58 AM
        0 responses
        103 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-05-2026, 10:09 AM
        0 responses
        125 views
        0 reactions
        Last Post SEQadmin2  
        Working...