Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • 1bioinfo
    Junior Member
    • Jun 2011
    • 1

    finding gene from scaffolds, no annotation available

    Hello,

    I have all scaffolds of a bird genome from Illumina sequencing platform, I blast it against chicken genome to find the genes. Since there are millions of scaffolds it takes too long to find and visualize the genes with Geneious Pro software, The annotation file for chicken is available but not for my bird of interest (we have only scaffolds and contigs).
    I appreciate if anyone can help me to find a very fast computational way for finding genes.

    Thanks
  • jeremyrualosr
    Junior Member
    • Mar 2011
    • 3

    #2
    Try Geneious Support and UserForum

    The support and development team at Biomatters can help you... post on the Geneious User Forum and email [email protected] with your intended workflow...I'll give it a go and let you know what I come up with...

    Comment

    • DZhang
      Senior Member
      • Jun 2010
      • 177

      #3
      Hi 1bioinfo,

      You may predict genes first in your contigs (http://www.cbcb.umd.edu/research/genefinding.shtml), and then use other tools (blast2go or blastx or iprscan) to annotate them. For a project of your size, it will involve tons of efforts...

      Best regards,
      Douglas

      Comment

      Latest Articles

      Collapse

      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 07-02-2026, 11:08 AM
      0 responses
      12 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      14 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      54 views
      0 reactions
      Last Post SEQadmin2  
      Working...