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  • Dr. Hybe
    Member
    • Jun 2009
    • 14

    Sequencing Amplicons

    Quick Question - what is a good way to sequence amplicons with a a wide range of insert sizes?
  • ECO
    --Site Admin--
    • Oct 2007
    • 1360

    #2
    Quick answer: pool them roughly in equimolar fashion, concatemerize, shear, treat like a shotgun library prep.

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