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Old 09-12-2011, 10:17 AM   #1
chea
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Location: rockville

Join Date: Jul 2010
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Default General coverage questions for NGS

How many flow cells do I need for sequencing a human genome with 30X converage (paired-end)?
Does the number of reads related with the number of lanes to use in a flow cell?

If I want to sequence 8 microbial genomes de novo, each of the 8 genomes is 10 Mbases in size.
How many 100bp paired-end reads will be needed to achieve 50X coverage for each of the genomes? Will you suggest multiplexed sequencing?
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