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09-12-2011, 10:17 AM
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Junior Member
Location: rockville Join Date: Jul 2010
Posts: 1
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General coverage questions for NGS
How many flow cells do I need for sequencing a human genome with 30X converage (paired-end)?
Does the number of reads related with the number of lanes to use in a flow cell? If I want to sequence 8 microbial genomes de novo, each of the 8 genomes is 10 Mbases in size. How many 100bp paired-end reads will be needed to achieve 50X coverage for each of the genomes? Will you suggest multiplexed sequencing? |
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| Tags |
| coverage calculation, illumina, paired end reads |
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