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Old 09-12-2011, 10:17 AM   #1
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Default General coverage questions for NGS

How many flow cells do I need for sequencing a human genome with 30X converage (paired-end)?
Does the number of reads related with the number of lanes to use in a flow cell?

If I want to sequence 8 microbial genomes de novo, each of the 8 genomes is 10 Mbases in size.
How many 100bp paired-end reads will be needed to achieve 50X coverage for each of the genomes? Will you suggest multiplexed sequencing?
chea is offline   Reply With Quote

coverage calculation, illumina, paired end reads

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