Tweet
I am a biologist and a poor programmer. I have assembled RNA-seq data from which I'd like to extract all potential long ncRNA candidates. I assume the easiest way would be to compare my data to Refseq or Rfam but I don't know how to do this. Furthermore, this strategy would only pick up already annotated lncRNA. I would be glad of any help.
-
Last edited by stephenhart; 09-28-2011, 11:45 PM. -
You can use Dario web server which is a free web tool for ncRNA analysis using RNAseq data.
I have not used it before. so can not comment on the results. Please let me know your experience wit it. -
You could do it with Annocript. It annotates the transcriptome and gives putative lnc RNAs sequences...Comment
-
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
Comment