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Hi all,
I'm Paul from Sydney. I am currently working on getting iCLIP protocol to work on my protein of interest. So far I am on the right track and I seem to be pulling down some RNA targets. I am about to enter this world of NGS which I know very little about.
Currently, my RNA sample has been converted into cDNA and has been tagged using Solexa primer sequence ready to go (following iCLIP protocol). However, I might have to change my primer sequences so that I can run my sample on Solid machines. Does anyone know where I can get more information about what primer sequences that Solid system uses?
I am looking forward to learning more from many of you about NGS and analysis of seq data.
Cheers,
Paul
I'm Paul from Sydney. I am currently working on getting iCLIP protocol to work on my protein of interest. So far I am on the right track and I seem to be pulling down some RNA targets. I am about to enter this world of NGS which I know very little about.
Currently, my RNA sample has been converted into cDNA and has been tagged using Solexa primer sequence ready to go (following iCLIP protocol). However, I might have to change my primer sequences so that I can run my sample on Solid machines. Does anyone know where I can get more information about what primer sequences that Solid system uses?
I am looking forward to learning more from many of you about NGS and analysis of seq data.
Cheers,
Paul