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I want to used cnvHMM but it requires a cns file. I am not sure what exactly a cns file is so if anyone has some knowledge, it would be appreciated.
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Last edited by dmacmillan; 01-11-2012, 09:26 AM. -
readme fiel not clear
Hi
The cnvNMM READMe file is not clear. I could not figure out the format or type of cnsfile. Some idea? -
I think cns file is the consensus read depth file.
Just came across samtools mpileup for Variant and SNP calls..
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thank you ganygan25Comment
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Hi,
I'm also trying to get this to work. Did samtools mpileup work? If so what did you try?
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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