Tweet
How many reads will be generated for each library in HiseqSQ sequencing for miRNAs? I want to know how many samples I can pool in one lane. Thank you every!!
-
-
I think your question is better phased as "how many reads can I get from one lane from a HiSeqSQ". I presume you are actually talking about the HiScanSQ. Then knowing how many reads you need per sample you will know how many samples you can pool. We got around 82M reads per lane in our last single-end run. Theoretical is a bit more. Sample prep will play a big role; our range was from 74M to 89M. -
Thank you for reply! So, how many reads can cover all the miRNAs for each library? I want to know the maximum number of libraries that can be pooled in a lane? How many libraries can generate 82M reads in your last single-end run. Thank you so much!!!!Originally posted by westerman View PostComment
-
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM -
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...07-09-2026, 11:10 AM -
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...07-08-2026, 05:17 AM
Comment