454 sample normalisation

[HMcC-TGAC]

Hello everyone,

Does anyone know what 1e7 molecules/ul is in nM?

I'm trying to normalise some samples and working in nM would be far easier.

Many thanks,

Heather.

[TonyBrooks]

Avagadro's constant is 6.022e23 (molecules per mole)
Therefore 1e7 would contain be 1.66e-17 moles in 1µL
or 1.66e-11 moles in 1L (i.e. Molar) --- 1e6 µL in 1L
or 16.6pM --- pico is 1e-12

[RPiddock]

Thats what I get too.