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I have a control and a knockdown experiment from which I am sequencing small rnas. When I count the sum of the reads mapped to the known mirnas foreach condition I get 968384 for contol anD 71736 for the knockdown. I really dont know how to interpret the results and if normalizing by the total nr of reads makes sence. Important I cannot use the assumption that the expression of most miRNAs should not change. Any suggestions?
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM