Post-expiry Illumina reagents

ilmns79 · 02-07-2012, 05:51 AM

Does anyone have experience using Illumina HiSeq v3 reagents after their expiry date? Everything is still stored at -20 in original packaging. Kits in question is SR and PE v3 clustering kits and sequencing kits. If so, how far out have you used them? Be aware, my Illumina products arrived with only 3 months left on the shelf life!

GW_OK · 02-07-2012, 06:02 AM

As long as it was stored properly, I don't see what could have gone bad....

TonyBrooks · 02-07-2012, 07:32 AM

I was told by my FAS that the SR kits are more robust to being used past expiry than the PE kits. I guess it has something to do with how the linearisation is achieved.
We have some SR kits that are about 6 months out of date and we're going to put them on in a few weeks to see if we get anything, but I have no info at the moment.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
emPCR Reagents	Anthony.287	454 Pyrosequencing	2	11-18-2011 11:35 AM
v3 Flowcells and Reagents?	NextGenSeq	Illumina/Solexa	7	06-28-2011 05:57 AM
Bad Illumina Reagents	aleferna	Illumina/Solexa	2	05-13-2011 06:56 AM
What happens when Illumina sequencing reagents run out?	picabo	Illumina/Solexa	2	01-17-2011 07:46 AM
Defective Sequencing Reagents to Cost Illumina up to $23M in 2009	odelfour	Illumina/Solexa	3	11-10-2009 03:22 PM

02-07-2012, 05:51 AM	#1
ilmns79 Junior Member Location: US Join Date: Feb 2012 Posts: 1	Post-expiry Illumina reagents Does anyone have experience using Illumina HiSeq v3 reagents after their expiry date? Everything is still stored at -20 in original packaging. Kits in question is SR and PE v3 clustering kits and sequencing kits. If so, how far out have you used them? Be aware, my Illumina products arrived with only 3 months left on the shelf life!

02-07-2012, 06:02 AM	#2
GW_OK Senior Member Location: Oklahoma Join Date: Sep 2009 Posts: 270	As long as it was stored properly, I don't see what could have gone bad....

02-07-2012, 07:32 AM	#3
TonyBrooks Senior Member Location: London Join Date: Jun 2009 Posts: 213	I was told by my FAS that the SR kits are more robust to being used past expiry than the PE kits. I guess it has something to do with how the linearisation is achieved. We have some SR kits that are about 6 months out of date and we're going to put them on in a few weeks to see if we get anything, but I have no info at the moment.