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Methyl-Analyzer--whole genome DNA methylation profiling.
Bioinformatics. 2011 Aug 15;27(16):2296-7
Authors: Xin Y, Ge Y, Haghighi FG
Abstract
SUMMARY: Methyl-Analyzer is a python package that analyzes genome-wide DNA methylation data produced by the Methyl-MAPS (methylation mapping analysis by paired-end sequencing) method. Methyl-MAPS is an enzymatic-based method that uses both methylation-sensitive and -dependent enzymes covering >80% of CpG dinucleotides within mammalian genomes. It combines enzymatic-based approaches with high-throughput next-generation sequencing technology to provide whole genome DNA methylation profiles. Methyl-Analyzer processes and integrates sequencing reads from methylated and unmethylated compartments and estimates CpG methylation probabilities at single base resolution. AVAILABILITY AND IMPLEMENTATION: Methyl-Analyzer is available at http://github.com/epigenomics/methylmaps. Sample dataset is available for download at http://epigenomicspub.columbia.edu/m...yzer_data.html.
CONTACT: [email protected]
SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
PMID: 21685051 [PubMed - indexed for MEDLINE]
More...
Methyl-Analyzer--whole genome DNA methylation profiling.
Bioinformatics. 2011 Aug 15;27(16):2296-7
Authors: Xin Y, Ge Y, Haghighi FG
Abstract
SUMMARY: Methyl-Analyzer is a python package that analyzes genome-wide DNA methylation data produced by the Methyl-MAPS (methylation mapping analysis by paired-end sequencing) method. Methyl-MAPS is an enzymatic-based method that uses both methylation-sensitive and -dependent enzymes covering >80% of CpG dinucleotides within mammalian genomes. It combines enzymatic-based approaches with high-throughput next-generation sequencing technology to provide whole genome DNA methylation profiles. Methyl-Analyzer processes and integrates sequencing reads from methylated and unmethylated compartments and estimates CpG methylation probabilities at single base resolution. AVAILABILITY AND IMPLEMENTATION: Methyl-Analyzer is available at http://github.com/epigenomics/methylmaps. Sample dataset is available for download at http://epigenomicspub.columbia.edu/m...yzer_data.html.
CONTACT: [email protected]
SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
PMID: 21685051 [PubMed - indexed for MEDLINE]
More...