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  • iamsong
    Junior Member
    • Feb 2012
    • 1
    #1

    De novo genome throughput !?!?!?

    Dear All:

    I want to sequence a de novo genome from a fish(about 1Gb) , using HiSeq200 system. How many coverage should I do, and I can have a draft map??

    My first step:
    500bp library in one 100PE lane
    2000bp library in one 100PE lane

    Depend on first step result estimating the K-mer and doing next step sequencing.

    Is the throughput enough for estimating the K-mer in my first step???
    How much throughput should I do???
    Should I use 454 system???

    Thanks a lot,
    SONG.
    Tags: de novo genome
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