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Old 02-13-2012, 07:52 AM   #1
iamsong
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Location: Taiwan

Join Date: Feb 2012
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Default De novo genome throughput !?!?!?

Dear All:

I want to sequence a de novo genome from a fish(about 1Gb) , using HiSeq200 system. How many coverage should I do, and I can have a draft map??

My first step:
500bp library in one 100PE lane
2000bp library in one 100PE lane

Depend on first step result estimating the K-mer and doing next step sequencing.

Is the throughput enough for estimating the K-mer in my first step???
How much throughput should I do???
Should I use 454 system???

Thanks a lot,
SONG.
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