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02-13-2012, 07:52 AM
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Junior Member
Location: Taiwan Join Date: Feb 2012
Posts: 1
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De novo genome throughput !?!?!?
Dear All:
I want to sequence a de novo genome from a fish(about 1Gb) , using HiSeq200 system. How many coverage should I do, and I can have a draft map?? My first step: 500bp library in one 100PE lane 2000bp library in one 100PE lane Depend on first step result estimating the K-mer and doing next step sequencing. Is the throughput enough for estimating the K-mer in my first step??? How much throughput should I do??? Should I use 454 system??? Thanks a lot, SONG. |
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