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|02-13-2012, 08:52 AM||#1|
Join Date: Feb 2012
De novo genome throughput !?!?!?
I want to sequence a de novo genome from a fish(about 1Gb) , using HiSeq200 system. How many coverage should I do, and I can have a draft map??
My first step:
500bp library in one 100PE lane
2000bp library in one 100PE lane
Depend on first step result estimating the K-mer and doing next step sequencing.
Is the throughput enough for estimating the K-mer in my first step???
How much throughput should I do???
Should I use 454 system???
Thanks a lot,
|de novo genome|