Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • vcc
    Junior Member
    • Apr 2011
    • 4

    differential expression analysis of microrna generated by NGS platform

    Hi all,
    I am very much new to this NGS technology. I have already used cufflinks for transcriptome analysis as a small project. But now I am looking to get differential expression analysis of microRNA single ended reads. So my question is will it be reasonable to use cufflinks on this data too?
    If not so, kindly can I get some explanation for the same.

    Hoping to get response soon.

    thanks all.
  • Xiaomin Yu
    Junior Member
    • Jul 2011
    • 4

    #2
    You could try cuffdiff if you make your own miRNA gtf file, or you can use miRdeep2

    Comment

    • gpcr
      Member
      • May 2010
      • 18

      #3
      How to use mirdeep2 for differential expression analysis

      1. How can i use mirdeep2 outputs for differential expression analysis?

      2. which files to use? results.csv or expression.csv?

      3. how to deal with duplicate mature miRNA(with different precursors?) in the output.

      thanks for your inputs.

      Comment

      Latest Articles

      Collapse

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Yesterday, 10:09 AM
      0 responses
      10 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-04-2026, 08:59 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-02-2026, 12:03 PM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-02-2026, 11:40 AM
      0 responses
      22 views
      0 reactions
      Last Post SEQadmin2  
      Working...