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low bead return from E120
Hello All, our last E120 only produced 700M beads (and not the expected ~2.2B). All of our templates looked good throughout the library process and we used qPCR to quantify before pooling for the emulsifier stage.
We have seen a low bead return before but that was when we tried to use the qubit concentrations for the final quantification so the low return was probably as a result of low template input.
Has anyone seen a low bead output that related to something other than low template input?
JPC
We have seen a low bead return before but that was when we tried to use the qubit concentrations for the final quantification so the low return was probably as a result of low template input.
Has anyone seen a low bead output that related to something other than low template input?
JPC
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