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Do i have to do any sorting on the BAM file that Tophat generates before i convert it to a bcf/vcf file (using Samtools)?
Thanks for your help.
Thanks for your help.
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Introduction
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