Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • guzhi100
    Member
    • Feb 2012
    • 15
    #1

    cuffdiff log2 value

    Hi,

    I am analyzing my RNA-Seq data. After running cuffdiff, I got a list of differentially expressed transcrpts or genes.

    As far as I know, log2 value = fold change. However, there are minus values. Is this possible?? log2 value can not be minus. Did I miss something??



    Looking forward to your help.

    Thanks in advance.

    Best
    Tags: None
    • kopi-o
      Senior Member
      • Feb 2008
      • 319
      #2
      The log2 value can most definitely be negative. Why not?

        Comment

        • sdriscoll
          I like code
          • Sep 2009
          • 436
          #3
          Try log2(0.5) and log2(2). Log2 scale is convenient.
          /* Shawn Driscoll, Gene Expression Laboratory, Pfaff
          Salk Institute for Biological Studies, La Jolla, CA, USA */

            Comment

            Previous template Next

            Latest Articles

            Collapse
            • SEQadmin2
              Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
              by SEQadmin2



              Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

              Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating              ...
              10-14-2026, 09:55 AM
            • SEQadmin2
              New Genomics Technologies Take Aim at Long-Standing Limits
              by SEQadmin2


              Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

              We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing              ...
              09-28-2026, 10:25 AM
            View All

            ad_right_rmr

            Collapse

            News

            Collapse
            Topics Statistics Last Post
            Mayo Clinic-Led Study Detects DNA Mutations Across Noncancerous Tissues by SEQadmin2
            Started by SEQadmin2, 10-15-2026, 10:52 AM
            0 responses
            23 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-15-2026, 10:52 AM  
            Study Finds Chromatin Motion Holds Steady Until Cells Face Stress by SEQadmin2
            Started by SEQadmin2, 10-14-2026, 05:39 AM
            0 responses
            27 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-14-2026, 05:39 AM  
            Larger Datasets Reveal Additional Switches That Control Gene Expression by SEQadmin2
            Started by SEQadmin2, 10-13-2026, 09:56 AM
            0 responses
            23 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-13-2026, 09:56 AM  
            Computational Tool Captures How Chromatin Folds at Scale by SEQadmin2
            Started by SEQadmin2, 10-09-2026, 11:14 AM
            0 responses
            28 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-09-2026, 11:14 AM  
            View All
            Working...