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Old 04-27-2012, 06:48 AM   #1
cerebralrust
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Location: Sweden

Join Date: Jan 2012
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Exclamation 1/4th of microtitre plate enough to characterize transcriptome of non-model legume?

Dear Members,

In your experience, do you think 1/4th of a microtitre plate is enough to characterize the genome of a non-model legume species?

The 454 sequencing performed in the above fashion only resulted in a few hundred thousand reads of a not-so great coverage.

I was under the impression that,to fully characterize a transcriptome, one must sequence several samples of many genotypes in illumina and 454, combine the data and assemble, annotate etc, to achieve a good quality transcriptome that may represent the species.

What do you think?
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