Anybody have this problem before? A brand new box of SV oil; one tube missing...
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Nope, but I've had leaky oil tubes and cups before... -
missing reagents
Haven't seen missing oil tubes....yet. But we did have this XLR70 kit the other day with no ATP. The tube wasn't cracked or leaking.....it was just empty!Comment
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We have a XL+ kit without DTT tube!!!!!!!!
I write to sequencing service and he replace immediately the kit.
...no comment!
do you check all the kits when you receive them?Comment
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We had a sleeve of FLX+ reagents (50mL tubes) where 2 of the reagents were in the wrong positions...
They replaced the kit without issue.Comment
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Interesting.
Yeah, I've started checking everything as soon as it shows up. Didn't think it would be necessary, but after seeing all of these replies I think differently.Comment
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two tubes in wrong position!!!!!
I will check the kits better now.
Do you have te lot number of this kit?Comment
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Double checking is a good idea - we're all human and mistakes happen. We struggled to find a list of reagents and their proper positions in any of the 454 manuals/reagent inserts which was a bit alarming. I understand they will include something like this in their next update to the manuals. Until then, make your own list
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Thought I would contribute - nothing dramatic but; my supervisor couldn't figure out why the sipper manifold wouldn't lower completely when he was doing a maintenance wash until he discovered.........Comment
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We had the following:
1) Polymerase at half the level required
2) Empty Apyrase Tupe
3) Cracked XL sequencing tray tubes (leaking)Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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