Hi,
I am new to NGS. I have question on bwa indexed files.
I installed bwa-0.6.1 onto our lab server. Then I index the PhiX genome as below,
$bwa index –a bwtsw /data/genomes/PhiX/Sequence/PhiX-bwa-build/chr.fa
I also tried
$bwa index –a is /data/genomes/PhiX/Sequence/PhiX-bwa-build/chr.fa
both ways generate five files, which are (.amb, .ann, .bwt, .pac, .sa files)
But in another server from another lab, bwa (could be different version, I don't know which one) indexed files has nine files
(.fai, .rpac, .amb, .ann, .pac, .bwt, .rbwt, .rsa, .sa nine files)
I am confused on this. Can somebody explain why it happens?
Then I run alignment using bwa on same ngs reads on both server, the .sam files generated on both servers are a little different.
What should I do to make it right? Thanks a lot.
I am new to NGS. I have question on bwa indexed files.
I installed bwa-0.6.1 onto our lab server. Then I index the PhiX genome as below,
$bwa index –a bwtsw /data/genomes/PhiX/Sequence/PhiX-bwa-build/chr.fa
I also tried
$bwa index –a is /data/genomes/PhiX/Sequence/PhiX-bwa-build/chr.fa
both ways generate five files, which are (.amb, .ann, .bwt, .pac, .sa files)
But in another server from another lab, bwa (could be different version, I don't know which one) indexed files has nine files
(.fai, .rpac, .amb, .ann, .pac, .bwt, .rbwt, .rsa, .sa nine files)
I am confused on this. Can somebody explain why it happens?
Then I run alignment using bwa on same ngs reads on both server, the .sam files generated on both servers are a little different.
What should I do to make it right? Thanks a lot.
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