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  • sbsusers
    Junior Member
    • Jun 2012
    • 3

    #1

    bwa indexed files?

    Hi,

    I am new to NGS. I have question on bwa indexed files.

    I installed bwa-0.6.1 onto our lab server. Then I index the PhiX genome as below,
    $bwa index –a bwtsw /data/genomes/PhiX/Sequence/PhiX-bwa-build/chr.fa

    I also tried
    $bwa index –a is /data/genomes/PhiX/Sequence/PhiX-bwa-build/chr.fa

    both ways generate five files, which are (.amb, .ann, .bwt, .pac, .sa files)

    But in another server from another lab, bwa (could be different version, I don't know which one) indexed files has nine files
    (.fai, .rpac, .amb, .ann, .pac, .bwt, .rbwt, .rsa, .sa nine files)

    I am confused on this. Can somebody explain why it happens?

    Then I run alignment using bwa on same ngs reads on both server, the .sam files generated on both servers are a little different.

    What should I do to make it right? Thanks a lot.
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    All the genomes I have indexed (with version 0.5.9), both human genome and small bacterial genomes, have the 8 files you list above. bwa doesn't make .fai files, samtools can.

    Maybe you have an older version of bwa, and you are accidently using that instead?

    Comment

    • sbsusers
      Junior Member
      • Jun 2012
      • 3

      #3
      Thank you for the reply, swbarnes2.

      Comment

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