Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Clean room or not? denice_please Core Facilities 2 05-21-2012 01:45 AM
Is there a program to error check / clean up FASTQ files? marcovth Bioinformatics 1 11-04-2011 07:29 AM
PCR Clean up - 96 well HGENETIC Sample Prep / Library Generation 0 07-26-2010 01:44 AM
help: how to clean pair-edn Ilumina reads before assembly hinsby Bioinformatics 5 03-02-2010 11:57 AM

Thread Tools
Old 07-02-2012, 10:01 AM   #1
Senior Member
Location: Massachusetts

Join Date: May 2009
Posts: 116
Default 96 well PCR clean up

Anyone found a 96 well clean up method that will remove 100 nt/bp and smaller from ~250 bp product? Most give 100 bp as the cutoff, but we've gotten too much small material back with Qiaquick 96. Alternative is Ampure, and it can be tricky to handle in 96 well plate. We'd like to clean up, quantitate, THEN pool and size select to get rid of rest of small material before sequencing. Have tried quantitation, pooling, then clean up and size selection, and dataset yields vary too much. Thanks; Hilary
HMorrison is offline   Reply With Quote
Old 07-02-2012, 04:55 PM   #2
Location: Maryland

Join Date: Apr 2010
Posts: 31

We've had success with the 96 Well PureLink Pro kit from Invitrogen

Hope this helps.
nexgengirl is offline   Reply With Quote
Old 07-02-2012, 06:32 PM   #3
--Site Admin--
Location: SF Bay Area, CA, USA

Join Date: Oct 2007
Posts: 1,358

Nothing will outperform AMPure IMHO. You'll probably have to titrate the cut (specifically the binding time and HOW you mix [static vs constant mixing]) in your own hands, but I would start with 1.3-1.4x for that separation range.
ECO is offline   Reply With Quote

amplicon sequencing, cleaning, pcr, primer dimer

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:53 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO