Tweet
There is some information in the original rumors thread...but I wanted to start a new thread to get some of the _actual_ post-V2-upgrade information out there (either from myself or others).
We are still in the middle of our 2x250 phiX install runs on 2 of our MiSeqs, everything has gone really nicely so far. The hardware upgrade took basically 8 hours spread over two days with two FSE's working on it.
Things I've noticed:
V1 intensity profile:
V2 intensity profile:
And %>Q30 looks similar but the higher quality in the V2 chemistry/imaging is evident comparing the quality at the same cycle...
V1 %>Q30 (where at C150 it's at ~80%)
V2 %>Q30 (where at C150 it's at 87%):
Also, in the interest of benchmarking relative cluster densities, we ran the same dilution of the same phiX library (12.5pM according to the FAS) on one of our V1 instruments as well. He recommended not doing this because the observed V1 cluster density was expected to be higher than for V2.
The two V2 instruments came up at 803 and 848...while the V1 run came up at 883. This is very close to our V1 experience, where we run 12pM to target 900K/mm^2, but it will be interesting to track if the moderately lower V2 cluster density is a real phenomenon.
Anyway...looking great so far...stay tuned...I hope to run some real samples as soon as possible.
We are still in the middle of our 2x250 phiX install runs on 2 of our MiSeqs, everything has gone really nicely so far. The hardware upgrade took basically 8 hours spread over two days with two FSE's working on it.
Things I've noticed:
- Flow cell part numbers are IDENTICAL between V1 and V2. Based on the part numbers on the external storage tube.
- The V2 instruments are ~40 cycles ahead of the V1 instrument (which is running 2x151)
- The intensity profiles are crazy different (see below)
V1 intensity profile:
V2 intensity profile:
And %>Q30 looks similar but the higher quality in the V2 chemistry/imaging is evident comparing the quality at the same cycle...
V1 %>Q30 (where at C150 it's at ~80%)
V2 %>Q30 (where at C150 it's at 87%):
Also, in the interest of benchmarking relative cluster densities, we ran the same dilution of the same phiX library (12.5pM according to the FAS) on one of our V1 instruments as well. He recommended not doing this because the observed V1 cluster density was expected to be higher than for V2.
The two V2 instruments came up at 803 and 848...while the V1 run came up at 883. This is very close to our V1 experience, where we run 12pM to target 900K/mm^2, but it will be interesting to track if the moderately lower V2 cluster density is a real phenomenon.
Anyway...looking great so far...stay tuned...I hope to run some real samples as soon as possible.
Comment