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How does new Truseq Stranded Protocol actually work? Is it dUTP based??
I have not done this work. Please advise. thank you very much.
I have not done this work. Please advise. thank you very much.
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Yes it is dUTP based. Also adds actinomycin D to first strand synthesis, presumably to prevent any second strand synthesis during that step.
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Phillip -
Philip, Thank you very much clarifying this. Did you use this in your laboratory? and how happy are you with this?
thank you.Comment
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Have a kit, did not try it yet. Next week, maybe.
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PhillipComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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