Second-strand RNA synthesis NEB

eab

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Join Date: May 2011

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#1

Second-strand RNA synthesis NEB

10-11-2012, 01:57 PM

For anyone who may use NEB's second strand cDNA enzyme mix in their RNA libraries -

Have you ever left your reactions in the thermocycler at 4 degrees overnight after completing? I am concerned because there's a DNA ligase in there - will large concatamers of insert form if I leave it on park overnight instead of returning to the lab at 9:00 to purify?

Thanks!
Eli
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NMD

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Join Date: Jun 2012

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#2

10-15-2012, 11:18 AM

The NEB Mastermix protocol makes a note to say that is fine.

-Nicolette
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