How to index a genome for BLAST

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Avro1986

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Join Date: Dec 2012

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#1

How to index a genome for BLAST

12-10-2012, 12:23 PM

Hi everyone!

I have just installed BLAST+ 2.2.27 on my mac. I want to run some cDNAs against arabidopsis thaliana's genome. I know I need to download the genome and format it for BLAST searches but I don't know how.

I would greatly appreciate any help.

Thank you very much for your time!
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ucpete

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#2

12-10-2012, 01:25 PM

makeblastdb -in <genome.fasta> -dbtype nucl
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Avro1986

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#3

12-10-2012, 01:49 PM

Thank you!
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